• Grazing incidence to total internal reflection fluorescence structured illumination microscopy enabled by a prism telescope 

      Ortkrass, Henning; Wiebusch, Gerd; Linnenbrügger, Jochen; Schürstedt, Jasmin; Szafranska, Karolina Joanna; McCourt, Peter Anthony; Huser, Thomas (Journal article; Tidsskriftartikkel; Peer reviewed, 2023-11-13)
      In super-resolution structured illumination microscopy (SR-SIM) the separation between opposing laser spots in the back focal plane of the objective lens affects the pattern periodicity, and, thus, the resulting spatial resolution. Here, we introduce a novel hexagonal prism telescope which allows us to seamlessly change the separation between parallel laser beams for 3 pairs of beams, simultaneously. ...
    • High-speed TIRF and 2D super-resolution structured illumination microscopy with a large field of view based on fiber optic components 

      Ortkrass, Henning; Schürstedt, Jasmin; Wiebusch, Gerd; Szafranska, Karolina Joanna; McCourt, Peter Anthony; Huser, Thomas Rolf (Journal article; Tidsskriftartikkel; Peer reviewed, 2023-08-16)
      Super-resolved structured illumination microscopy (SR-SIM) is among the most flexible, fast, and least perturbing fluorescence microscopy techniques capable of surpassing the optical diffraction limit. Current custom-built instruments are easily able to deliver two-fold resolution enhancement at video-rate frame rates, but the cost of the instruments is still relatively high, and the physical size ...